| Rebekka M. Wachter Publications |
28. Pouwels, L. J., Zhang, L., Chan, N. H., Dorrestein, P. C., Wachter, R. M. (2008) Kinetic
Isotope Effect Studies on the de novo Rate of Chromophore Formation in Fast- and Slow-
maturing GFP Variants. Biochemistry, in press.
27. Malo, G. D., Wang, M., Wu, D., Stellin, A., Tonge, P. J., Wachter, R. M. (2008) Crystal
Structure and Raman Studies of dsFP483, a Cyan Fluorescent Protein from Discosoma striata.
J. Mol. Biol. 378, 869-884.
26. Malo, G. D., Pouwels, L. J., Wang, M., Weichsel, A., Montfort, W. R., Rizzo, M. A., Piston,
D. W., Wachter, R. M. (2007). X-ray Structure of Cerulean GFP: A Tryptophan-Based
Chromophore Useful for Fluorescence Lifetime Imaging. Biochemistry 46, 9865-9873.
Accelerated Publication, Featured as “Hot Article”, Featured on the journal’s Most Accessed
Articles website.
25. Wachter, R. M. (2007). Chromogenic Cross-link Formation in Green Fluorescent
Protein. Acc. Chem. Res. 40, 120-127. Invited Review.
24. Zhang, L., Patel, H. N., Lappe, J. W., Wachter, R. M. (2006). Reaction Progress of
Chromophore Biogenesis in Green Fluorescent Protein. J. Am. Chem. Soc. 128, 4766-4772.
23. Wachter, R. M. (2006). The family of GFP-like proteins: Structure, Function,
Photophysics and Biosensor Applications. Introduction and Perspective, Symposium-in-print,
Photochem. Photobiol. 82, 339-344. Invited Review.
22. Wachter, R. M. (2006). Mechanistic Aspects of GFP Chromophore Biogenesis.
Progress in Biomedical Optics and Imaging 7. Proc. of SPIE Vol. 6098, 609803-1 – 609803-8.
Keynote Paper.
21. Wang, M., Patel, H. N., Wachter, R. M. (2005). X-ray Diffraction Analysis and Molecular
Replacement Solution of the Cyan Fluorescent Protein dsFP483. Acta Cryst. F61, 922-924.
20. Sniegowski, J. A., Phail, M. E., Wachter, R. M. (2005). Maturation Efficiency, Trypsin
Sensitivity and Optical Properties of Arg96, Glu222 and Gly67 Variants of Green Fluorescent
Protein. Biochem. Biophys. Res. Comm. 332, 657-663.
19. Scruggs, A. W., Flores, C. L., Wachter, R. M., Woodbury, N. W. (2005). Development
and Characterization of Green Fluorescent Protein Mutants with Altered Lifetimes. Biochemistry
44, 13377- 13384.
18. Rosenow, M. A., Patel, H. N, Wachter, R. M. (2005). Oxidative Chemistry in the GFP
Active Site Leads to Covalent Cross-Linking of a Modified Leucine Side Chain with a Histidine
Imidazole: Implications for the Mechanism of Chromophore Formation. Biochemistry 44, 8303-
8311.
17. Sniegowski, J. A., Lappe, J. W., Patel, H. N., Huffman, H. A., Wachter, R. M. (2005).
Base-catalysis of Chromophore Fromation in Arg96 and Glu222 variants of Green Fluorescent
Protein. J. Biol. Chem. 280, 26248-26255.
16. Remington, S. J., Wachter, R. M., Yarbrough, D. K., Branchaud, B. P., Anderson, D. C.,
Kallio, K., Lukyanov, K. A. (2005). zFP538, a Yellow Fluorescent Protein from Zoanthus,
Contains a Novel Three-Ring Chromophore. Biochemistry 44, 202-212. “Top ten most cited” for
the year 2005.
15. Rosenow, M. A., Huffman, H. A., Phail, M. E., Wachter, R. M. (2004). The Crystal
Structure of the Y66L Variant of Green Fluorescent Protein Supports a Cyclization-Oxidation-
Dehydration Mechanism for Chromophore Maturation. Biochemistry 43, 4464-4472.
14. Bell, A. F., Stoner-Ma, D., Wachter, R. M., Tonge, P. J. (2003). Light Driven
Decarboxylation of Wild-Type Green Fluorescent Protein. J. Am. Chem. Soc. 125, 6919-6926.
13. Yarbrough, D., Wachter, R. M., Kallio, K., Matz, M. V., Remington, S. J. (2001). Refined
Crystal Structure of DsRed, a Red Fluorescent Protein from Coral, at 2.0 Å Resolution. Proc.
Natl. Acad. Sci. USA 98, 462-467.
12. Wachter, R. M., Yarbrough, D., Kallio, K., Remington, S. J. (2000). Crystallographic and
Energetic Analysis of Binding of Selected Anions to the Yellow Variants of Green Fluorescent
Protein. J. Mol. Biol. 301, 159-173.
11. Bell, A. F., He, X., Wachter, R. M., Tonge, P. J. (2000). Probing the Ground State
Structure of the Green Fluorescent Protein Chromophore Using Raman Spectroscopy.
Biochemistry 39, 4423-4431.
10. Jayaraman, S., Haggie, P., Wachter, R. M., Remington, S. J., Verkman, A. S. (2000).
Mechanism and Cellular Applications of a Green Fluorescent Protein-based Halide Sensor. J.
Biol. Chem. 275, 6047-6050.
9. Wachter, R. M., Remington, S. J. (1999). Sensitivity of the YFP Form of Green
Fluorescent Protein to Halides and Nitrate. Curr. Biol. 9, R628-R629.
8. Elsliger, M.-A., Wachter, R. M., Kallio, K., Hanson, G. T., Remington, S. J. (1999).
Structural and Spectral Response of Green Fluorescent Protein Variants to Changes in pH.
Biochemistry 38, 5296-5301.
7. Wachter, R. M., Elsliger, M.-A., Kallio, K., Hanson, G. T., Remington, S. J. (1998).
Structural Basis of Spectral Shifts in the Yellow-emission Variants (YFPs) of Green Fluorescent
Protein. Structure 6, 1267-1277.
6. Wachter, R. M., Branchaud, B. P. (1998). Construction and Analysis of a Semi-
quantitative Energy Profile for the Reaction Catalyzed by the Radical Enzyme Galactose
Oxidase. Biochim. Biophys. Acta 1384, 43-54.
5. Wachter, R. M., King, B. A., Heim, R., Kallio, K., Tsien, R. Y., Boxer, S. G., Remington,
S. J. (1997). Crystal Structure and Photodynamic Behavior of the Blue-emission Variant
Y66H/Y145F of Green Fluorescent Protein. Biochemistry 36, 9759-9765.
4. Wachter, R. M., Montague-Smith, M. P., Branchaud, B. P. (1997). -Haloethanol
Substrates as Probes for Radical Mechanisms for Galactose Oxidase. J. Am. Chem. Soc. 119,
7743-7749.
3. Wachter, R. M., Branchaud, B. P. (1996). Thiols as Mechanistic Probes for Catalysis by
the Free Radical Enzyme Galactose Oxidase. Biochemistry 35, 14425-14435.
2. Wachter, R. M., Branchaud, B. P (1996). Molecular Modeling Studies on Oxidation of
Hexopyranoses by Galactose Oxidase. An Active Site Topology Apparently Designed to
Catalyze Radical Reactions, Either Concerted or Stepwise. J. Am. Chem. Soc. 118, 2782-
2789.
1. Montague-Smith, M. P., Wachter, R. M., Branchaud, B. P. (1992). Preparation of Fully
Oxidized Active and Reduced Inactive Forms of Galactose Oxidase from Dactylium dendroides
Using Ferricyanide-containing Oxidizing and Ferrocyanide-containing Reducing Forms of Ion
Exchange Resins. Anal. Biochem. 207, 353-355.
Department of Chemistry and Biochemistry
Center for Bioenergy and Photosynthesis
Center for Bioenergy and Photosynthesis
